solimena group
Antje Petzold
PhD project Antje
Cell biology of Enteroviruses and T1DT1D is caused by a combination of genetic and environmental factors, which may include viruses. It has been reported that infections in childhood with Human Enteroviruses (HEVs) such as Coxsackievirus B (CVB) and Echovirus can trigger or precipitate the onset of T1D (Andreoletti et al, 1997; Clemens et al, 1995; Hyoty et al, 1995). HEVs are non-enveloped, single stranded RNA viruses belonging to the family of Picornaviruses. They can cause acute clinical manifestations but mostly result in milder symptoms such as common cold, eye infection and skin disease or are asymptomatic (Roivainen, 2005). However, it is not know with certainty if and how HEVs is involved in the development of T1D. The aim of my project is to investigate the fate of mature SG proteins in insolinoma cells upon HEV infection. To address this question cells will be treated with inhibitors of the proteasome, lysosomes and autophagy. Furthermore, the expression of MHC class I molecules upon HEV infection will be examined and presented peptides will be identified and quantified by mass spectrometry. In addition, the ability of the identified peptides to activate a specific CD8+ T-cell line will be assessed.
Contact details:
antje.petzold {at} mailbox.tu-dresden.de
+49 (0)3 51 79 63 66 06