solimena group
Maria Grazia Magro
REGULATION OF GRANULE BIOGENESIS BY PTB1 IN MICE
The mRNA binding protein Polypyrimidine Tract-Binding protein 1 (PTB1) plays a key role in the posttranscriptional regulation of insulin granule biogenesis. Stimulation of beta cells with glucose or Glucagon-Like Peptide (GLP-1) leads to Protein Kinase A-dependent phosphorylation and nucleocytoplasmic translocation of PTB1. Activated cytosolic PTB1 binds to the 3’- and 5’-UTR of mRNAs encoding various granule proteins, increasing their stability and translation, and thus upregulating granule biogenesis.
Aim of this project is to assess the role of PTB1 in insulin granule biogenesis in vivo. Mice expressing a PTB mutant allele that lacks the ability to translocate to the cytosol, or a PTB mutant costitutively residing in the cytosol, will be generated using a transgenic approach. Characterization of these mice will allow to further dissect the respective roles of nuclear and cytosolic PTB1 in insulin granule biogenesis.
Contact details:
maria-grazia.magro {at} mailbox.tu-dresden.de
+49 (0)351 458.6652